CaspACE FITC-VAD-FMK In Situ Marker from Promega

Product Specs
ItemCaspACE FITC-VAD-FMK In Situ Marker
CompanyPromega
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberG7461
TargetMulti-caspase
Quantity50microl
MethodFluorometric (FITC)
Molecular Weight~86kDa

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) The splicing regulators TIA1 and TIAL1 are required for the expression of the DNA damage repair machinery during B cell lymphopoiesisCell reportsDecember 20, 2022Osma-Garcia IC, Capitan-Sobrino D, Mouysset M, Aubert Y, Maloudi O, Turner M, Diaz-Muñoz MD.proteins","Chemicals, peptides, and recombinant proteins" ,, CaspACE™ FITC-VAD-FMK In Situ Marker,Promega,Cat# G7461 Zombie NIR Fixable Viability dye,BioLegend,Cat# BLE423106 Recombinant Murine IL-7,Peprotech,Cat# 217-17-10ug KU55933,Sigma

(2) Fungal Bioreporters to Monitor Outcomes of Aspergillus: Host–Cell InteractionsMethods in Molecular BiologyApril 27, 2022Neta Shlezinger, Jeffrey Scott Fites, Bruce S. Klein, Tobias M. HohlAssays Phosphate-buffered saline (PBS). TWEEN-20. FITC-VAD-fmk (CaspACE in situ marker, Promega Corp., G7461). Anti-CD45 Ab (optional; to distinguish fungal and mammalian cells). FACS buffer (PBS with 0.5%

(3) Epithelial dynamics shed light on the mechanisms underlying ear canal defectsDevelopmentDecember 1, 2020Juan M. Fons, Mona Mozaffari, Dean Malik, Abigail R. Marshall, Steve Connor, Nicholas D. E. Greene, Abigail S. Tuckercultured in DMSO at the same concentration (1.25%). In situ CaspACE FITC-Z-VAD-FMK marker (Promega, G7461) was added to the culture medium in the last day of culture for 30 min under the same normal culture

(4) Precise Expression of Afmed15 Is Crucial for Asexual Development, Virulence, and Survival of Aspergillus fumigatusmSphereOctober 28, 2020Luyu Guan, Ruiyang Lu, Zhengjun Wu, Guowei Zhong, Shizhu Zhangcaspase activity. The caspase in situ labeling fluorescence analysis system for the FITC–VAD-fmk probe (G7461; Promega Corp., Madison, WI, USA) was used to stain the activity of fungal caspase as previously described,

(5) BIK drives an aggressive breast cancer phenotype through sublethal apoptosis and predicts poor prognosis of ER-positive breast cancerCell death & diseaseJune 11, 2020Pandya V, Githaka JM, Patel N, Veldhoen R, Hugh J, Damaraju S, McMullen T, Mackey J, Goping IS.Resulting cell pellets were resuspended in 200 μL growth medium containing 10 μM CaspAce reagent (#G7461, Promega, USA) or just growth medium for unstained control and incubated in the cell-culture incubator

(6) Mitochondrial damage by α-synuclein causes cell death in human dopaminergic neuronsCell death & diseaseNovember 14, 2019Ganjam GK, Bolte K, Matschke LA, Neitemeier S, Dolga AM, Höllerhage M, Höglinger GU, Adamczyk A, Decher N, Oertel WH, et al.Following 72 h of AAV2 viral infection, cells were incubated with medium containing 5 µM of FITC-VAD-FMK (G7461, Promega, Madison, USA) for 30 min at 37 °C. Cells were briefly washed with PBS and imaged immediately

(7) 3-MA Inhibits Autophagy and Favors Long-Term Integration of Grafted Gad67–GFP GABAergic Precursors in the Developing Neocortex by Preventing ApoptosisCell TransplantationNovember 1, 2014Christian Roux, Céline Lesueur, Caroline Aligny, Carole Brasse-Lagnel, Damien Genty, Stéphane Marret, Annie Laquerrière, Soumeya Bekri, Bruno J. Gonzalezfluorescein isothiocyanate N-benzyloxycarbonyl-Val-Ala-Asp(O-Me) fluoromethyl ketone (FITC-VAD-FMK) in situ marker (G7461), and the ApoONE homogeneous caspase-3/7 kit (G7760) were from Promega (Charbonnieres les Bains, France).

(8) Acute humanin therapy attenuates myocardial ischemia and reperfusion injury in miceArteriosclerosis, thrombosis, and vascular biologyOctober 1, 2010Muzumdar RH, Huffman DM, Calvert JW, Jha S, Weinberg Y, Cui L, Nemkal A, Atzmon G, Klein L, Gundewar S, et al.cell death, 1nM HNG was added 30min prior to the addition of daunorubicin. CaspACE FITC-VAD-FMK (cat# G7461, Promega Corporation, was next used in an assay for apoptosis in H9c2 cells. This FITC conjugated cell

(9) Alterations of the Medullary Epithelial Compartment in the Aire-Deficient Thymus: Implications for Programs of Thymic Epithelial DifferentiationThe Journal of ImmunologyOctober 15, 2008James Dooley, Matthew Erickson, Andrew G. Farrprocedures in the absence of TdT served as a negative control. A fluorescent pan-caspase inhibitor (cat.# G7461, Promega, Madison WI) was mixed with primary antibodies at a final concentration of 2.5 µM, using tissue

(10) Endogenously generated hydrogen peroxide induces apoptosis via mitochondrial damage independent of NF-?B and p53 activation in bovine embryosTheriogenologyJanuary 1, 2007Carlos Velez-Pardo, Ariel Tarazona Morales, Marlene Jimenez Del Rio, Martha Olivera-Angel

(11) Akt Decreases Lymphocyte Apoptosis and Improves Survival in SepsisThe Journal of ImmunologyJune 15, 2004Ursula Bommhardt, Katherine C. Chang, Paul E. Swanson, Tracey H. Wagner, Kevin W. Tinsley, Irene E. Karl, Richard S. Hotchkisswere resistant to apoptosis, the CaspACE FITCVAD-FMK in situ marker kit (Promega, Madison, WI; catalog G7461) was used, as per the manufacturer’s instructions. Flow cytometry detection of Akt and active caspase 3

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